Monday, April 22, 2013

Typing Our Own Blood


Last class, we performed an experiment using synthetic blood and antiserums to practice typing blood. This class, we carried out a similar procedure, except this time, we used our own blood!

First, we obtained the following materials:

1 Antiserum containing blood typing card
1 Beaker of water
1 Pipet
1 Cover sheet
1 Lancet
4 Eldon sticks
1 Alcohol Swab
1 Cotton ball

This is the procedure we followed:
1.       Fill in the necessary information on your blood typing card.


2.       Activate the antiserums on the blood typing card by adding one drop of water to each section.


3.       Wipe your finger with the alcohol swab, and use the lancet to pierce through the skin.


4.       Scoop up the blood with an Eldon stick, and mix in the blood with the first antiserum on the card. Add blood to all of the antiserums using different Eldon sticks each time.


5.       Press the cotton ball to your finger to stop the bleeding.
6.       See which of the blood samples agglutinated on the card, and figure out your blood type.
If the blood with the anti-A serum agglutinated, you are type A.
If the blood with the anti-B serum agglutinated, you are type B.
If the blood with the anti-A serum and the anti-B serum both agglutinated, you are type AB.
If neither the blood with the anti-A serum and the anti-B serum agglutinated, you are type O.
If the blood with the anti-D serum agglutinated, you are Rh+. If it didn’t, you are Rh-.
7.       Write your blood type on your card, and place a cover sheet over it once it dries.




If you are type O-, you are a universal donor.
If you are type AB+, you are a universal acceptor.

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